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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Leuk Lymphoma. 2012 Dec 31;54(6):1288–1296. doi: 10.3109/10428194.2012.752485

Figure 2. Cellular apoptosis is induced after combination treatment.

Figure 2

Figure 2

Figure 2

(A) Patient leukemic NK cells from 6 patients were treated with 12.5 µM C6-Ceramide and 5, 10, or 15 µM PPMP for 24 hrs then stained for 7AAD//7-AAD then assayed for apoptosis by flow cytometry. (B) RNK-16 cells were treated with 6.25 µM C6-Ceramide nanoliposomes and 5, 10, or 15 µM of PPMP (n=3 independent experiments), then apoptosis assay was performed. (C) NKL were treated with 12.5 µM C6-Ceramide and 5, 10, or 15 µM PPMP (n=3 independent experiments), then apoptosis assay was performed. (D) Normal PBMCs were treated with 12.5 µM C6-Ceramide nanoliposomes and 5, 10, or 15 µM PPMP (n=8), then apoptosis assay was performed. *, P<0.05, **, P<0.005, ***, P<0.0005 indicate significant differences of C6-Ceramide nanoliposomes+PPMP versus control treated cells (Student’s t-test).