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. 2014 Dec 23;3:e04591. doi: 10.7554/eLife.04591

Video 5. Lattice light sheet movie of an embryo expressing GFP::MEG-3 and mCherry::PGL-3.

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DOI: 10.7554/eLife.04591.025

Time-lapse of the posterior cytoplasm of a zygote (anterior to the left, posterior to the right) acquired using lattice light sheet microscopy in dithered mode. Two fusing granules are highlighted (also shown in Figure 5D). Images are maximum intensity projections of 11 z planes separated by 0.3 μm steps, capturing the entire depth of the fusing pair. Stacks were taken every 580 ms, total movie time is 34 s, movie is played back in 5× real time.

DOI: http://dx.doi.org/10.7554/eLife.04591.025