Table 5.

Differential expression of protein families associated with lignocellulolytic activity in enriched thermophilic and mesophilic communities

Pfam	Name	Overexpressed in 1	Fold change over other community	P -value, pseudo-Pfam comparison method 4,5	Adjusted P -value, DESEQ method 5
Pfam00150	GH5	M	2.73	0.960	1.000
Pfam00232	GH1	T	9.33	0.333	0.331
Pfam00331	GH10	T	6.90	0.540	0.369
Pfam00457	GH11	T	7.53	0.109	0.369
Pfam00722	GH16	M	2.57	0.769	1.000
Pfam00759	GH9	T	4.79	0.542	0.569
Pfam00840	GH7	T	n/a2	0.291	0.256
Pfam00933	GH3	M	1.26	0.970	1.000
Pfam01270	GH8	T	1.28	0.975	1.000
Pfam01341	GH6	T	59.68	0.152	0.018
Pfam01670	GH12	T	20.49	0.623	0.466
Pfam01915	GH3C	M	1.39	0.864	1.000
Pfam02011	GH48	T	129.95	0.105	0.012
Pfam02015	GH45	M	n/a2	0.751	1.000
Pfam02156	GH26	M	7.94	0.890	0.878
Pfam03443	GH61	T	113.00	0.364	0.323
Pfam03648	GH67N	M	1.10	0.859	1.000
Pfam03664	GH62	T	42.87	0.282	0.093
Pfam04616	GH43	M	1.16	0.946	1.000
Pfam07477	GH67C	M	1.06	0.840	1.000
Pfam07488	GH67M	M	1.15	0.667	1.000
Pfam00553	CBM2	T	30.07	0.097	0.012
Pfam00734	CBM1	n/a3	n/a3	n/a3	n/a3
Pfam00942	CBM3	T	29.40	0.179	0.221
Pfam02013	CBM10	M	n/a2	0.485	0.093
Pfam02018	CBM4,9	M	1.47	0.247	1.000
Pfam03067	CBM33	T	49.61	0.028	0.016
Pfam03422	CBM6	M	2.31	0.930	1.000
Pfam03424	CBM17,28	M	n/a2	0.956	1.000
Pfam03425	CBM11	M	1.27	0.988	1.000
Pfam03426	CBM15	M	n/a2	0.656	1.000
Pfam09212	CBM27	n/a3	n/a3	n/a3	n/a3
Pfam09478	CBM49	n/a3	n/a3	n/a3	n/a3

¹T, thermophilic community; M, mesophilic community.

²Fold change cannot be calculated because read count is 0 in one metatranscriptome.

³Pfam expression not detected in either metatranscriptome.

⁴ P-value is the mean from running the algorithm three times.

⁵Bold values indicate P-values ≤ to the critical value of 0.1.