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. Author manuscript; available in PMC: 2015 Jun 12.
Published in final edited form as: Science. 2014 Dec 12;346(6215):1377–1380. doi: 10.1126/science.1259530

Fig. 3. Reduced particle motion and improved resolution on ultra-stable grids.

Fig. 3

(A) 80S ribosomes were tracked during electron irradiation, using the same imaging conditions, for particles supported on three different am-C grids (black circles (17)) and three different gold grids (gold crosses). Each point is the in-plane ensemble average displacement of a particle from its initial position, and the error bars are the s.e.m. for the multiple grids tested. Solid lines are linear fits to the two phases of motion, where the slopes are the average speeds of the particles. There was a 43% reduction in the speed of the first phase (8.8 Å/s to 5.0 Å/s) and a 77% reduction in the speed of the second phase (2.3 Å/s to 0.53 Å/s) on gold relative to am-C. Density maps of apoferritin processed identically from data collected using identical conditions for am-C (B) and gold (C) substrates. Map (B) has a resolution of 25 Å (gold-std. FSC) and contains no discernible information beyond that present in the initial model. Compare to (C) with a resolution of 8.0 Å which resolves the entire molecular chain.