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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Gut. 2010 Jun 28;59(8):1066–1078. doi: 10.1136/gut.2009.203893

Figure 8.

Figure 8

Granulocyte-macrophage colony stimulating factor (GM-CSF) regulates the survival and proliferation of intestinal epithelial cells through activation of STAT5. (A) Subconfluent Caco-2 cells were stimulated with rhGM-CSF (1 ng/ml) for 15, 30 and 60 min and the activation of STAT5 (pSTAT5) was examined in nuclear proteins (NE) by western blot analysis. (B) STAT5, Bid, Bax and cleaved caspse-3 expression were measured by western blot analysis with and without STAT5 a and b siRNA (STAT5 siRNA). Control (CON) group: transfection reagent without siRNA. (C) Caco-2 cells were stimulated by rhGM-CSF (1 ng/ml) with and without STAT5 siRNA and cell proliferation was measured using BrdU incorporation. *p<0.05 vs control group. (D) Cyclin D1 and p21 were identified by western blot analysis with and without STAT5a and b RNAi. Signal intensity was determined by densitometry and is shown as the mean±SEM. Results representative of five independent experiments are shown.