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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Anal Biochem. 2014 Jun 2;461:36–45. doi: 10.1016/j.ab.2014.05.018

Table 1.

MurX-catalyzed syntheses of lipid I analogues from N-glycolyl- and N-acetyl-Park’s nucleotides

graphic file with name nihms-617284-t0004.jpg
Entry1 Park’s nucleotide Decaprenyl phosphate
(equivalents against 1 or 2)		 Lipid I Reaction
time (h)		 Yield (%)2
1 1 2 3 1 15-20
2 1 2 3 3 15-20
3 1 10 3 1 20-25
4 2 2 4 1 15-20
5 2 2 4 3 15-25
6 2 10 4 1 20-25
1

Reaction conditions: Park’s nucleotide (2 mM; 3.75 μL), MgCl2 (0.5 M; 10 μL); KCl (2 M, 10 μL), Triton X100 (0.1%; 11.25 μl), Tris-buffer (pH = 8; 50mM, 5 μL), decaprenyl phosphate (10 mM, 2 or 10 equivalents against 1 or 2), P-60 (15 μL), 26 °C, 1 or 3h.;

2

n-butanol extract was analyzed by HPLC (column: Kinetex 5u C8 100A, 150×4.60 mm, solvent: CH3CN : 0.05 M aq. NH4HCO4, flow rate: 0.5 mL / min.