. Author manuscript; available in PMC: 2015 Jan 16.

Published in final edited form as: Anal Biochem. 2014 Jun 2;461:36–45. doi: 10.1016/j.ab.2014.05.018

Table 2.

Assay of positive- and negative-controls, and a library of uridyl peptides against MurX



entry	Compound	MurX inhibition (%)^a					IC₅₀ (μM)^b

		0.01 μM	0.1 μM	1 μM	10 μM	100 μM
1	Capuramycin (7)	-	36.6	88.4	100	100	0.152 ± 0.0125	(0.150-0.0180)^c
2	SQ641 (8)	-	40	95	100	-	0.109 ± 0.00845	(0.0150-0.100)^c
3	Tunicamycin (9)	-	9	42	72	72	2.73 ± 0.138	(2.40-2.95)^c
4	10	-	0	0	0	0	ND
5	11	-	0	0	0	0	ND
6	Vancomycin	-	0	0	0	0	ND
7	12	3.8	81.2	100	100	-	0.105 ± 0.00330
8	13	-	40	95	100	-	0.0950 ± 0.00395
9	14	-	0	0	0	0	ND
10	15	-	0	0	0	0	ND
11	DMSO	0	0	0	0	0	ND

1) Reaction conditions: Park’s nucleotide-N^ε-dansylthiourea 2 (75 μM; 3.75 μL), MgCl₂ (0.5 M; 10 μL); KCl (2 M, 10 μL), Triton X100 (0.1%; 11.25 μl), Tris-buffer (pH = 8; 50 mM, 2.5 μL), neryl phosphate 6 (10 mM, 45 μL), inhibitor molecule (0-100 μM in DMSO (2.5 μL)), P-60 (15 μL), 26 °C, 3h.; The K_m 18.29 μM (Park’s nucleotide 2).

The IC₅₀ values were obtained three times and the standard error of the mean was calculated.

The IC₅₀ values in parentheses were reported in the literatures (see, references 18, 19, 24, 25, 45) and/or were obtained via the assay conditions summazried in Table 1.; Reaction conditions: Park’s nucleotide-N^ε-dansylthiourea 2 (75 μM; 3.75 μL), MgCl₂ (0.5 M; 10 μL); KCl (2 M, 10 μL), Triton X100 (0.1%; 11.25 μl), Tris-buffer (pH = 8; 50 mM, 5 μL), decaprenyl phosphate (10 mM, 2 equivalents against 2), inhibitor molecule (0-100 μM in DMSO (2.5 μL)), P-60 (15 μL), 26 °C, 3h.; The K_m 18.05 μM (Park’s nucleotide 2).