Table 2.
Inter-assay variability (RSD) measured across three proficiency testing rounds and summarized by clinical pharmacology laboratory, antiretroviral and concentration level (n = 1 result per level per analyte per laboratory per round).
| CPL | ATV
|
DRV
|
EFV
|
FTC
|
RGV
|
RTV
|
TFV
|
Median CPL RSD
|
CPL Min/Max
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H | L | H | L | H | L | H | L | H | L | H | L | H | L | H | L | H&L | |
| Target concentration (ng/ml) | 7000 | 1500 | 7000 | 1500 | 9000 | 1500 | 2500 | 1250 | 4500 | 900 | 2500 | 500 | 240 | 120 | |||
|
| |||||||||||||||||
| 1 | 3.29 | 2.79 | 1.54 | 3.32 | 4.77 | 1.02 | 2.84 | 2.77 | - | - | 4.34 | 3.70 | 2.25 | 2.46 | 3.07 | 2.78 | 1.02/4.77 |
|
| |||||||||||||||||
| 2 | 2.44 | 3.50 | 3.93 | 3.67 | 3.67 | 1.84 | 3.39 | 1.98 | 7.04 | 4.06 | 2.12 | 4.53 | 5.89 | 3.04 | 3.67 | 3.50 | 1.98/7.04 |
|
| |||||||||||||||||
| 3 | - | - | - | - | 6.76 | 7.07 | - | - | 6.10 | 1.95 | 2.81 | 4.63 | - | - | 6.10 | 4.63 | 1.95/7.07 |
|
| |||||||||||||||||
| 4 | - | - | - | - | - | - | 2.79 | 21.5† | - | - | - | - | 5.08 | 5.59 | 3.94 | 13.5 | 2.79/21.5 |
|
| |||||||||||||||||
| 5 | 5.05 | 1.17 | 4.32 | 2.62 | 6.07 | 3.52 | - | - | - | - | 6.48 | 7.48 | - | - | 5.56 | 3.07 | 1.17/7.48 |
|
| |||||||||||||||||
| 6 | 3.05 | 3.45 | 4.20 | 2.96 | 1.57 | 2.78 | 23.3‡ | 8.79 | - | - | 3.22 | 5.32 | - | - | 3.22 | 3.45 | 1.57/23.3 |
|
| |||||||||||||||||
| 7 | 3.09 | 6.24 | 5.99 | 8.46 | 6.85 | 5.03 | 7.01 | 7.28 | - | - | 4.13 | 5.20 | 4.98 | 4.71 | 5.49 | 5.72 | 3.09/8.46 |
|
| |||||||||||||||||
| 8 | 4.08 | 3.67 | - | - | 0.38 | 0.96 | - | - | - | - | 3.10 | 3.21 | - | - | 3.10 | 3.21 | 0.38/4.08 |
|
| |||||||||||||||||
| 9 | 4.95 | 3.13 | 5.39 | 3.09 | 5.24 | 0.78 | 5.38 | 3.09 | 2.64 | 3.67 | 4.82 | 1.83 | 2.16 | 3.36 | 4.95 | 3.09 | 0.78/5.39 |
|
| |||||||||||||||||
| 10 | 7.45 | 7.25 | 7.85 | 10.6 | 2.35 | 0.65 | 4.12 | 3.81 | 2.75 | 2.37 | - | - | 7.13 | 6.45 | 5.62 | 5.13 | 0.65/10.6 |
|
| |||||||||||||||||
| Median ARV, RSD | 3.68 | 3.47 | 4.32 | 3.32 | 4.77 | 1.84 | 4.12 | 3.81 | 4.42 | 3.02 | 3.67 | 4.58 | 5.03 | 4.03 | Median H | Median L | Overall Median |
|
| |||||||||||||||||
| Min/Max | 1.17/7.25 | 1.54/10.6 | 0.38/7.07 | 2.77/23.3 | 2.75/7.04 | 1.83/7.48 | 2.16/7.13 | 4.32 | 3.48 | 3.69 | |||||||
Laboratory failed 1/3 rounds for proficiency; please note that normal QC will have passed.
Laboratory was outside 20% acceptance for 2/3 values but still passed proficiency at 80%; please note that normal QC will have passed.
Values >15% are formatted as bold and underlined.
ARV: Antiretroviral; ATV: Atazanavir; CPL: Clinical pharmacology laboratory; DRV: Darunavir; EFV: Efaviren; FTC: Emtricitabine; H: high; L: low; LPV:Lopinavir; RGV: Raltegravir; RPD: Relative percentage difference; RSD: Relative standard deviation; RTV: Ritonavir; TFV: Tenofovir.