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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Aug 7;1843(11):2796–2806. doi: 10.1016/j.bbamcr.2014.07.019

FIGURE 3. Requirement of Exon 9 in the Anti-apoptotic Effect of E2.

FIGURE 3

(A) Using a single monoclonal anti-ERα antibody from Abcam that recognizes all three splice variants, ERα66, ERα46, and ERα36, western blots were performed on whole cell lysates from wildtype HEK293 cells (293wt), and HEK293 transiently transfected to overexpress wildtype ERα36 cDNA (293ovrx36) and exon 9-deleted ERα36 cDNA (293ex9d36). (B) PLD activity is reduced with 10−8M E2 wildtype HEK293 cells, while E2 increases PLD activity in ERα36 overexpressed HEK293 cells. In cells overexpressing exon 9-deleted ERα36, E2 did not exhibit this effect. * represents p<0.05 compared to the corresponding untreated control group. (C) ERα36 overexpression mediated the anti-apoptotic effect of E2 against taxol-induced caspase-3 activity, but in cells with exon 9-deleted ERα36, this effect was not evident. * represents p<0.05 compared to the corresponding untreated 293wt, # represents p<0.05 compared to taxol only in 293wt, $ represents p<0.05 compared to untreated 293ovrx36, and @ represents p<0.05 compared to taxol only in 293ovrx36.