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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Am J Transplant. 2012 Feb 2;12(5):1102–1112. doi: 10.1111/j.1600-6143.2011.03959.x

Figure 2. BALB specific alloimmunization after depletion and BMT.

Figure 2

(A) BALB specific alloantibody production. anti-BALB antibodies in sera of transfused and transplanted recipients were assessed using BALB.B splenocyte (white) and platelet (grey) targets in an indirect immunofluorescence staining. (B) In vivo survival of BALB.B targets. Immunity against BALB expressing targets was assessed by in vivo survival of BALB.B splenocyte targets labeled with CFDA. Error bars in (A) represent the mean + SEM. The mean of each group in (B) is represented as a horizontal line. Representative histograms are shown to illustrate typical outcomes of the in vivo clearance assay. Statistics were generated using column statistics and a one-way ANOVA with Dunnett’s post-test. The data shown for both panels A and B is the combined data from three separate experiments.