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. 2015 Jan 9;8:49–67. doi: 10.2147/CEG.S70119

Table S1.

TaqMan gene expression assays employed to monitor aquaporin expression

Gene product Substrate ABI assay IDa Interrogated sequence RefSeq Translated protein RefSeq NCBI location chromosome Exon boundary
AQP1 H2O Hs00166067_m1b NM_198098.1 NP_932766.1 Chr. 7 – 30951415–30965131 1–2, assay loc: 492
AQP3 H2O
Glycerol
H2O2
NH4+
Urea
Hs00185020_m1 NM_004925.3 NP_004916.1 Chr. 9 – 33441158–33447590 2–3 assay loc: 297
AQP4 H2O Hs00242342_m1 NM_001650.4 NP_001641.1 Chr. 18 – 24432007–24445716 2–3 assay loc: 508
AQP5 H2O Hs00387048_m1 NM_001651.2 NP_001642.1 Chr. 12 – 50355279–50359461 1–2 assay loc: 888
AQP7 H2O
Glycerol
Urea
Hs00357359_m1 NM_001170.1 NP_001161.1 Chr. 9 – 33384948–33402517 2–3 assay loc: 199
AQP8 H2O
H2O2
NH4+
Hs00154124_m1 NM_001169.2 NP_001160.2 Chr. 16 – 25228285–25240253 2–3 assay loc: 339
AQP9 H2O
Glycerol
Urea
Hs00175573_m1 NM_020980.3 NP_066190.2 Chr. 15 – 58430408–58478110 1–2 assay loc: 467
TNF-α Hs99999043_m1 NM_000594.2 NP_000585.2 Chr. 6 – 31543350–31546112 1–2 assay loc: 360
IL-23A Hs00372324_m1 NM_016584.2 NP_057668.1 Chr. 12 – 56732663–56734194 1–2 assay loc: 323
COX-2/PTGS2 Hs00153133_m1 NM_000963.2 NP_000954.1 Chr. 1 – 186640944–186649559 5–6 assay loc: 777

Notes:

a

All assays used dye label FAM;

b

An assay whose probe spans an exon junction and does not detect genomic DNA.

Abbreviations: HS, Homo sapiens; AQP, aquaporin; IL, interleukin; loc, location; COX-2, cyclooxygenase 2; TNF-α, tumor necrosis factor-α.