Table S3.

Comparison of aquaporin mRNA levels from CD and UC patients and non-IBD patient controls in the small and large bowel biopsies

	CD vs controls			CD vs UC			UC vs controls
	mRNA ratio	P-value	q-value	mRNA ratio	P-value	q-value	mRNA ratio	P-value	q-value
Ileum
AQP1	0.54	0.07	0.13	0.43	<0.001	0.002	1.28	0.37	0.61
AQP3	0.15	<0.001	0.003	0.16	<0.001	<0.001	0.94	0.87	0.87
AQP7	1.16	0.67	0.67	4.31	0.002	0.006	0.27	0.003	0.02
AQP8	7.33	0.29	0.36	260.96	0.005	0.01	0.03	0.07	0.17
COX-2	2.56	0.075	0.13	0.57	0.31	0.38	4.51	0.014	0.05
TNF-α	2.94	0.06	0.13	4.00	0.01	0.02	0.73	0.62	0.69
IL-23A	4.43	0.03	0.10	6.93	0.01	0.017	0.64	0.56	0.69
Right colon
AQP1	0.83	0.72	0.72	0.90	0.98	0.98	0.84	0.78	0.78
AQP3	0.82	0.63	0.72	1.26	0.53	0.77	0.65	0.40	0.45
AQP7	1.97	0.37	0.59	10.98	<0.001	0.001	0.18	0.06	0.17
AQP8	0.25	0.03	0.27	52.46	0.008	0.04	0.01	0.001	0.009
COX-2	1.41	0.21	0.51	0.32	0.02	0.051	4.35	0.003	0.02
TNF-α	0.76	0.68	0.72	2.06	0.07	0.12	0.37	0.17	0.31
IL-23A	2.57	0.21	0.51	1.17	0.77	0.87	2.19	0.27	0.35
Left colon
AQP1	0.87	0.77	0.86	1.06	0.84	0.84	0.82	0.67	0.77
AQP3	0.54	0.09	0.34	0.78	0.31	0.40	0.70	0.30	0.40
AQP7	1.09	0.86	0.86	11.65	<0.001	<0.001	0.09	<0.001	<0.001
AQP8	0.08	0.004	0.03	247.47	<0.001	0.002	0.0003	<0.001	<0.001
COX-2	1.60	0.37	0.49	0.16	0.003	0.007	9.81	<0.001	<0.001
TNF-α	2.08	0.26	0.42	2.17	0.03	0.06	0.96	0.95	0.95
IL-23A	2.66	0.13	0.43	0.79	0.58	0.65	3.36	0.06	0.12

Note: mRNA levels detected by qRT-PCR are given as mRNA ratios calculated from delta delta C_T values with P-values and multiple testing adjusted P-values (q-values).

Abbreviations: AQP, aquaporin; CD, Crohn’s disease; COX-2, cyclooxygenase 2, IBD, inflammatory bowel disease; IL, interleukin; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; TNF-α, tumor necrosis factor-α; UC, ulcerative colitis; vs, versus.