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. 2015 Jan 16;10(1):e0116633. doi: 10.1371/journal.pone.0116633

Figure 3. FTI-277 treatment reversed burn-induced attenuated insulin-stimulated phosphorylation of Akt and GSK-3β, and glucose uptake in skeletal muscle.

Figure 3

Protein expression of Akt and GSK-3β was not altered by burn, FTI-277 or insulin (B, G). Insulin-stimulated phosphorylation of Akt at threonine 308 and serine 473 was blunted at 3 days after burn compared with sham-burn, which was reversed by FTI-277 treatment (C-F). Likewise, insulin-stimulated phosphorylation of GSK-3β at serine 9 was decreased in burned mice, which was reversed by FTI-277 treatment (H, I). n = 5 mice per group for saline-injected mice, n = 6 mice per group for insulin-injected sham-burned mice, n = 8 mice per group for insulin-injected burned mice. (J) Insulin-stimulated glucose uptake was attenuated by burn injury, and FTI-277 restored it in burned mice. n = 4 mice per group for sham-burned mice, n = 6 mice per group for burned mice. *p<0.05, **p<0.01, ***p<0.001, NS: not significant.