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Markers of cartilage degeneration and inflammation detected in the medium of cartilage plugs 24 hours after being impacted at different impact loads. (A) Glycosaminoglycan (GAG) was measured using the DMMB-based Blycan assay. (B) Prostaglandin E2 (PGE2) was measured by ELISA. (C) Nitrite release was measured using the Griess reagent system (NED reduction). Values are the mean ± SD of 13 (A) or 9 (B and C) samples from three independent experiments (*, P < 0.01).
