Skip to main content
. 2012 Jul;3(3):267–277. doi: 10.1177/1947603511435273

Figure 3.

Figure 3.

Fibronectin (FN) cleavage by MMP-1, -3, -13, and -14 at acidic pH 5.5. The fibronectin fragments (FN-fs) were measured at time intervals of 0.1, 2, 4, and 24 hours from Western blot analysis of FN digestion by each MMP. The molecular weights were quantified by Totallab TL100 densitometry (Nonlinear Dynamics, Durham, NC) based on intact FN (220 kDa) and FN-fs (70 kDa and 30 kDa) standards of known molecular weight incubated without MMPs for 24 hours (lane 1, CTRL). (A) Western blot analysis using a mouse monoclonal antibody specific for an epitope located within the fourth-type 3 repeat of human plasma FN. (B) Western blot analysis using a rabbit polyclonal anti-human plasma FN antibody. (C) The disappearance of the intact FN subunit expressed as a percentage of the FN remaining. No FN-fs were detected after FN incubation with MMP-1 and -14.