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Time course of the appearance of the fibronectin fragments (FN-fs) generated by incubation of intact fibronectin (FN) with (A) MMP-3 and (B) MMP-13 at acidic pH 5.5. The results are expressed as the amount of each FN-f at 0.1, 2, 4, and 24 hours as quantified by Totallab TL100 densitometry (Nonlinear Dynamics, Durham, NC) relative to the initial amount of FN (lane 1, CTRL, Fig. 3). No FN-fs were generated by MMP-1 and -14.
