Figure 5.

Relative gene fold induction of human chondrocytes in the in vitro PEGDA system treated with IL-1β (CCM) for 3 weeks (n = 3). (A) Sustained IL-1β treatment of OA chondrocytes stimulated down-regulation of anabolic matrix genes (Col2, Col1, Agg) and up-regulation of catabolic genes (MMP-3, MMP-13, NF-κB1) when compared to the control (no IL-1β). Recovering trend exhibited when cellsexposed to IL-1β for only 3 days. (B) Expression of anabolic and catabolic genes of normal human chondrocytes stimulated with sustained IL-1β exhibited similar trends as OA cells but with larger fold inductions.