Table 1.
Primer Sequences for qPCR
| Genes | Human: sequences (5′-3′) | Bovine: sequences (5′-3′) | ||
|---|---|---|---|---|
| β-actin | F-TGGCACCACACCTTCTACAATGAGC53 | 54 °C | F-GGCACCCAGCACAATGAA | 54 °C |
| R-GCACAGCTTCTCCTTAATGTCACGC | R-GCTAACAGTCCGCCTAGAAGC | |||
| Aggrecan | F-CACGATGCCTTTCACCACGAC46 | 54 °C | F-CATCGGGCTTGCCAGAGTT | 54 °C |
| R-TGCGGGTCAACAGTGCCTATC | R-ACTGGTGTCCACGAACGTAATG | |||
| F-GCCTTGAGCAGTTCACCTTC54 | ||||
| R-CTCTTCTACGGGGACAGCAG | ||||
| Type I collagen | F-AGGGCCAAGACGAAGACATC55 | 55 °C | F-GGGCAACAGCAGATTCACTTAC | 54 °C |
| R-AGATCACGTCATCGCACAACA | R-CAAGGATAGGCAGGCGAGAT | |||
| Type II collagen | F-GAAACCATCAATGGTGGCTTCC56 | 54 °C | F-GCAACCCTGGAACTGATGGA | 54 °C |
| R-CGATAACAGTCTTGCCCCACTT | R-GCTCACCCGTTTGACCTTTT | |||
| Sox-9 | F-CCCCAACAGATCGCCTACAG57 | 54 °C | ND | NA |
| R-GAGTTCTGGTCGGTGTAGTC | ||||
| F-TTCATGAAGATGACCGACGA58 | ||||
| R-CACACCATGAAGGCGTTCAT | ||||
| MMP1 | F-CTGAAGGTGATGAAGCAGCC59 | 51 °C | ND | NA |
| R-AGTCCAAGAGAATGGCCGAG | ||||
| MMP2 | F-GCGACAAGAAGTATGGCTTC59 | 54 °C | ND | NA |
| R-TGCCAAGGTCAATGTCAGGA | ||||
| MMP3 | F-CTCACAGACCTGACTCGGTT59 | 54 °C | F-TCTGACGGTCTGGGAGGAG | 54 °C |
| R-CACGCCTGAAGGAAGAGATG | R-AGGTCCATCAAAAGGCAAAA | |||
| MMP13 | F-CTATGGTCCAGGAGATGAAG59 | 54 °C | F-GCTCACGCTTTCCCTCCT | 54 °C |
| R-AGAGTCTTGCCTGTATCCTC | R-CAAACTCATGGGCAGCAACA | |||
| TIMP1 | F-GACGGCCTTCTGCAATTCC60 | 51 °C | ND | NA |
| R-GTATAAGGTGGTCTGGTTGACTTCTG | ||||
| NF-κB1 | F-GCACGACAACATCTCATTGG61 | 54 °C | F-TTACAAAACCAGCCTCCGTG | 54 °C |
| R-CCCAAGAGTCATCCAGGTC | R-GCCGAAACTGTCCGAGAAA | |||
Note: ND = not done; NA = not applicable.
Second pairs of human aggrecan and Sox-9 primers used only for Figure 1 normal chondrocytes real-time PCR. All other PCR for human aggrecan and Sox-9 used first pairs of primers listed.