Figure 1.

(A) Expression and (B) production of RANKL2 from the 293 cells and 293RANKL2 cells as determined by semiquantitative PCR and Western blot, respectively. (C) RANKL2 protein modulation in 293RANKL2 (n = 5) cells incubated with vitamin D₃ (50 nM), TNF-α (5 ng/mL), and PTH (100 nM). Cells were incubated for 72 hours in the presence of the factors, and RANKL2 protein levels were determined in the cell lysates using a specific ELISA. Data are expressed over the control, which was attributed a value of 1. Statistical analysis was assessed by the Student t test, and the P value is as indicated.