Table 3. Primer sequences for REDVR assay.
Primer_orientation | Sequence |
---|---|
38_Forward (FAM) | 5′-AAGACTGAGCCACCAAGCAT-3′ |
38_Reverse | 5′-CTCCCTGGCACTTCAGAGTC-3′ |
234_Forward | 5′-TTTTGCAGTGGACACAGGAC-3′ |
234_Reverse (HEX) | 5′-GGGTTGCAAATCCAGAGAAA-3′ |
The primer pair 38 distinguishes FCGR3A from FCGR3B while the primer pair 234 distinguishes the HNA1a region from HNA1b.