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. 2015 Jan 16;10(1):e0117091. doi: 10.1371/journal.pone.0117091

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© 2015 Kawamoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic of the experiment. Hearts were perfused with TMRE, and the signals were observed using two-photon laser microscopy. The images were obtained serially from hearts perfused with buffer containing vehicle or CCCP (0.1 or 1 μM). (B) Representative images of TMRE signals. Scale bar: 50μm. (C) CCCP decreased the TMRE signals of perfused hearts in a dose-dependent manner 20 min after CCCP perfusion (n = 3–4 per group). *P < 0.05 versus vehicle.