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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Proteomics. 2014 Sep 22;15(0):310–317. doi: 10.1002/pmic.201400187

Figure 2. The circadian SILAC workflow.

Figure 2

Design of the SILAC proteomics experiment. Workflow followed for SILAC MS analysis of mouse liver protein extracts. As described in the text, so-called heavy mice were obtained by feeding the animals with a diet containing the 13C6-substituted version of lysine over 2 generations. Heavy mice were used as quantitative reference to be compared to normal mice, under restricted feeding during the dark phase, which were sampled every three hours during two days. This approach allows following quantitative proteome expression measurement with MS at protein and PTM level over the period under study.