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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2014 Nov 5;14(1):225–235. doi: 10.1158/1535-7163.MCT-14-0194

Figure 1. ΔNP63α positively regulates the expression of CXCR4 mRNA.

Figure 1

SUM-102 cells were infected with an empty adenovirus or an adenovirus encoding ΔNP63α. A) Left - Immunoblot detection of ΔNP63α and CXCR4 protein expression from SUM-102 protein lysates 24 hours post infection. Right - Relative mRNA expression of CXCR4 from RNA lysates 24 hours post infection. B) SUM-102 cells were transfected with a siRNA against ΔNP63α or scrambled control (20nM). Relative mRNA expression levels for Left) ΔNP63α and Right) CXCR4 was determined via qRT-PCR analysis of RNA lysates 48 hours post transfection. C) IMEC and SUM-102 cells were fixed and immunoflourescent analysis was performed to detect the expression of ΔNP63α and CXCR4 protein. 40× magnification. Data are means ± S.D.; and performed in triplicate (n=3), P-values are indicated.