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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2014 Nov 21;14(1):141–150. doi: 10.1158/1535-7163.MCT-14-0658

Figure 1. Confirmation of HER2 expression as a target for NIR-PIT in SKOV-luc cells.

Figure 1

(A) Expression of HER1 and HER2 in SKOV-luc cells was examined with FACS. HER2 was overexpressed more than HER1. Specific binding was demonstrated with a blocking study. (B) SKOV-luc cells were incubated with tra-IR700 for 6 hr, and observed with a microscope before and after irradiation of NIR light (2 J/cm2). Necrotic cell death was observed after exposure to NIR light (1 hr after PIT). Bar = 50 µm. (C) Membrane damage and necrosis induced by NIR-PIT was measured by dead cell count using PI staining. Cell killing increased in a NIR-light dose-dependent manner. (D) Luciferase activity in SKOV-luc cells was measured as relative light unit (RLU), which also decreased in a NIR-light dose-dependent manner. (E) Bioluminescence imaging (BLI) of a 10 cm dish demonstrated that luciferase activity in SKOV-luc cells decreased in a NIR-light dose-dependent manner.