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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Leukemia. 2014 Jul 16;29(2):423–432. doi: 10.1038/leu.2014.221

Figure 2. Inhibition of CRM1 with Leptomycin B reduces Hoxa cluster expression in CALM-AF10 cells.

Figure 2

(A) MEFs stably expressing empty vector or CALM-AF10 were treated with 0.7 nM LMB for 2.5 h, and Hoxa transcript levels were measured by real time qRT-PCR. Results are normalized to GAPDH and then to untreated empty vector MEFs by the ΔΔCt method. Statistical analysis was performed by one-way ANOVA for each Hoxa gene followed by Dunnett’s multiple comparison test; *P<.01. (B) Murine CALM-AF10 or (C) Hoxa9/Meis1 leukemia cells were grown in the absence or presence of LMB (0.7 nM, 2.5 h). Hoxa transcript levels were measured by qRT-PCR. Results are normalized to GAPDH and then to untreated cells by the ΔΔCt method. Statistical analysis was performed by Student’s t-test; *P<0.05. (D) ChIP analysis of di-methylated H3K79 in the promoter regions of Hoxa cluster genes in murine CALM-AF10 leukemia cells. Results were generated by qRT-PCR and are shown as a percent of input. For all panels, results are shown as mean ± SEM compiled from at least 3 separate experiments.