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. Author manuscript; available in PMC: 2015 Jan 17.
Published in final edited form as: Nat Genet. 2010 Aug 8;42(9):794–800. doi: 10.1038/ng.641

Figure 4. Cbl mutant proteins exhibit prolonged protein turnover and are associated with increased phosphorylated EGFR levels upon EGF stimulation.

Figure 4

Panel (a) HEK293 cells transfected with plasmids encoding EGFR in combination with HA-Cbl(WT) or HA Cbl(p.Y371H) were serum starved for 18 h followed by 15 min EGF (50 ng/ml) stimulation. Cells were then washed and maintained in serum-free media for the indicated periods of time. Equal amounts of whole cell extracts were resolved on SDS-PAGE and immunoblotted with the indicated antibodies. Panel (b) HEK293 cells transfected with plasmids encoding EGFR in combination with HA-Cbl(WT) or HA-Cbl(p.Y371H) were serum starved for 18 h followed by 15 min EGF (50 ng/ml) stimulation. Cells were then washed and maintained in serum-free media with (+) or without (-) MG132 for the indicated periods of time. Equal amounts of whole cell extracts were resolved on SDS-PAGE and immunoblotted with the indicated antibodies.

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