Immunohistochemical (IHC) for identification of CD74+ cells and immunofluorescent (IF) staining for identification of CD68+ cells in urinary bladder at 72 h after AIC induction (n = 6). A to C. Microscopic (200x) finding of IHC staining for number of CD74+ cells (black arrows) infiltrated in bladder among three groups. D. Analytical results: *vs. other group with different symbols, p < 0.0001. Scale bars in right lower corner represent 50 µm. E to G. Microscopic (200x) finding of IF staining for number of CD68+ cells infiltrated in bladder among three groups. H. Analytical results: *vs. other group with different symbols, p < 0.0001. Scale bars in right lower corner represent 50 µm. All statistical analyses using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡) indicate significance (at 0.05 level). SC = sham control; AIC = acute interstitial cystitis; ECSW = extracorporeal shock wave.