Figure 5.

Protective effect in Col10a1-Runx2 mice upon OA induction. A. After repeated intra-articular TGFβ1 injection and treadmill overrunning, the representative mouse knee-joint of WT mouse at 12 weeks’ age was subjected to India ink staining. Clear rough joint surface/shallow grooves (top, white arrow) and signs of synovitis (bottom, white arrow) were observed. B. Compared to the WT mice, the corresponding joint surface of TG mice show smooth and shiny appearance and no signs of synovitis were shown (white arrows). C. H&E staining of WT mouse knee joints subjected to TTR treatment showed fibrotic remodeling (majorly cartilages erosions and fibrosis) at the margins of joint surfaces (black arrow). D. Compared to WT mice, the corresponding joint surface of TG mice did not show fibrotic remodeling as shown in WT mouse joints (black arrow).