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Primer sequences (all 5’-3’) used in real-time RT-PCR
| Genes | GeneBank accession no | Forward primer | Reverse primer |
|---|---|---|---|
| IGF1R | NM_000875 | GGACAGGTCAGAGGGTTTC | CTCGTAACTCTTCTCTGTGCC |
| FOXO1 | NM_002015 | TGGACATGCTCAGCAGACATC | TTGGGTCAGGCGGTTCA |
| E2F1 | NM_005225 | CAGAGCAGATGGTTATGGT | TATGGTGGCAGAGTCAGT |
| IKKα | NM_001278 | TGGAGTTAGAGGCTGTGA | GTCATCTGCTCGCTGTAT |
| ACTB | NM_001101 | GTCCACCGCAAATGCTTCTA | TGCTGTCACCTTCACCGTTC |
