Table 2.
Comparison of different cell culture conditions for the in vitro culture of EBEC
| Matrix coating medium | Collagen I | Collagen IV | Collagen I + collagen IV | Collagen G | Fibronectin + BSA + collagen I | Fibronectin + BSA + collagen IV |
|---|---|---|---|---|---|---|
| BEGM | (+) | (+) | (+) | (+) | - | - |
| LHC-8 | +(+) | ++ | + | + | - | - |
| DMEM/F12 + 2% Ultroser | +(+) | +++ | ++ | +(+) | - | - |
| DMEM/F12 + 10% FBS | - | - | - | - | - | - |
| 50% BEGM +50% DMEM/F12 | + | + | + | + | - | - |
| 50% LHC-8 + 50% DMEM/F12 | + | (+) | + | (+) | - | - |
No growth - ; limited growth for 10 days only (+) ; growth during 10 days only + ; growth during the entire culture period ++/+++.