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. 2014 Dec 31;5:30. doi: 10.1186/s13100-014-0030-4

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© White et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic of L1Hs droplet digital PCR (ddPCR) assays. (A) A 5’-FAM labeled Taqman™ probe specific to the 5’ UTR of L1Hs is paired with an L1 5’ UTR-specific primer. The probe and primer anneal to complementary DNA strands. This primer/probe set can be used in conjunction with a unique genomic flanking primer to detect the 5’-insertion junction of specific full-length L1 elements in the human genome using ddPCR. A control assay using a primer and 5’-VIC labeled probe set specific to a housekeeping gene (RPP30) can be used in parallel to determine copy number. (B) A 5’-FAM labeled Taqman™ probe specific to the 3’ end of L1Hs is paired with an L1Hs-specific primer. The probe and primer anneal to complementary DNA strands. This primer/probe set can be used in conjunction with a unique genomic flanking primer to detect the 3’ insertion junction of specific L1Hs elements in the human genome using ddPCR.