Table 2.

N -glycan microheterogeneity of human GGT1 expressed in Pichia pastoris as determined by LC-MS/MS (see Figure 4 )

hGGT1
N -glycosylation		Theoretical	Mass	MS/MS
Site	N -glycan	Mass	Measured	Support
		[M + 3H] + 3
N120	Man8GlcNAc2	1131.1307	1131.1153	✓
	Man9GlcNAc2	1185.1483	1185.1520	✓
	Man10GlcNAc2	1239.1659	1239.1576	✓
	Man11GlcNAc2	1293.1835	1293.1609	✓
	Man12GlcNAc2	1347.2011	1347.1885	✓
	Man13GlcNAc2	1401.2187	1401.2055	✓
		[M + 3H] + 3
N344	Man7GlcNAc2	985.7410	985.7221	✓
	Man8GlcNAc2	1039.7586	1039.7490	✓
	Man9GlcNAc2	1093.7762	1093.7745	✓
	Man10GlcNAc2	1147.7938	1147.7869	✓
	Man11GlcNAc2	1201.8114	1201.8104	✓
	Man12GlcNAc2	1255.829	1255.8137	✓
		[M + 3H] + 3
N511	Man8GlcNAc2	1112.8331	1112.8189
	Man9GlcNAc2	1166.8507	1166.8450	✓
	Man10GlcNAc2	1220.8683	1220.8614	✓
	Man11GlcNAc2	1274.8859	1274.8865	✓
	Man12GlcNAc2	1328.9035	1328.8865	✓
	Man13GlcnNAc2	1382.9211	1832.9140	✓