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. 2015 Jan 6;8:1. doi: 10.1186/s13071-014-0608-1

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© Sanin and Mountford; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sm16 is enriched in pellet fraction of cercarial E/S products. Three preparations of 0-3hRP were fractionated by ultracentrifugation and the pellet fractions restored to the original volume of each preparation. (A) Protein content of each fraction expressed as a percentage of the total protein present in the original preparation. Bars = mean + SEM; statistical significance was tested using two tailed t-test (*** = p < 0.001). (B) SDS PAGE gel of 0-3hRP fractions (5, 10 and 20 μg) stained for protein. Black arrows highlight bands identified by mass spectrometry. (C) Equivalent volumes of 0-3hRP_S (78 μg) and 0-3hRP_P (10 μg) based on the original preparation, were processed for Western blot analysis alongside rSm16 (1 μg) probed using rabbit anti-rSm16 antibody, estimated as relative concentration of Sm16 / μg 0-3hRP. Bars are mean + SEM, two tailedt-test show statistically significant differences (**** = p < 0.0001).