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. 2015 Jan 6;8:1. doi: 10.1186/s13071-014-0608-1

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© Sanin and Mountford; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Uptake of fluorescently labelled rSm16 by BMMΦs. Representative confocal images of (A) BMMΦs exposed to labelled rSm16^AF594 (red) stained with DAPI (blue) within EEA-1⁺ endosomes (green) 10 min and 100 min after ligand stimulation. (B) Insert showing EEA-1⁺ endosomes containing rSm16^AF594 (2 μm x 2 μm) (C) BMMΦs exposed for 100 min to rSm16^AF594 (red) and DEXTRAN^FITC (green) washed and imaged after 10, 60 and 100 min. (63x objective, scale bar = 10 μm; acquired using a Zeiss LSM 710 invert microscope).