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. 2015 Jan 13;16(4):406–415. doi: 10.22074/cellj.2015.487

Table 1.

Primers for determination of mice markers using reverse transcription-polymerase chain reaction (RT-PCR)


Gene Primer sequence (5´- 3´) Product size (bp) Accession number

c-Kit F: CTCACATAGCAGGGAGCACA 123 NM_001122733.1
R: ACAACTCACCCACACGCATA
Nanog F: CTGCTCCGCTCCATAACTTC 97 NM_028016.2
R: GCTTCCAAATTCACCTCCAA
Fragilis F: AGCCTATGCCTACTCCGTGA 112 NM_025378.2
R: GAGGACCAAGGTGCTGATGT
Oct4 F: GTTCTCTTTGGAAAGGTG 147 NM_001252452.1
R: GCATATCTCCTGAAGGTT
Mvh F: GCTCAAACAGGGTCTGGGAAG 145 NM_010029.2
R: GCTTGATCAGTTCTCGAG
Scp3 F: CCGCTGAGCAAACATCTAAAGATG 161 NM_011517.2
R: GGAGCCTTTTCATCAGCAACAT
Dazl F: AAGGCAAAATCATGCCAAAC 72 NM_010021.4
R: TCCTGATTTCGGTTTCATCC
Zp3 F: TAACCGTGTGGAGGTACCCA 136 NM_011776.1
R: CAGGCGAAGAGAGAAAGCCA
Gapdh F: ACACCCACTCCTCCACCTTTG 112 NM_002046.4
R: TCCACCACCCTGTTGCTGTAG