Fig 3.

Quantitative analysis of pluripotency and neural stem cell (NSC) marker expression following exogenous Oct4 expression and VPA along with different small molecules including of BIX-01294, Bay K8644, RG-108 in the tissue collected from the rims of injected brain ventricles. A. Endogenous Oct4 expression, B. Nanog expression, C. Klf4 expression, D. c-Myc expression, E. Sox2 expression, F. Pax6 expression and G. Sox1 expression in different groups. Results were normalized by GAPDH as a housekeeping gene. Vehicle; 7 days PBS i.c.v injection, OV; Oct4 expressing lentivirus i.c.v injection followed by doxycycline i.c.v injections and VPA oral gavages for another 7 consecutive days, OBiBaV; Oct4 expressing lentivirus i.c.v injection followed by BIX, Bay k and doxycycline i.c.v injections and VPA oral gavages for another 7 consecutive days, OBiRV; Oct4 expressing lentivirus i.c.v injection followed by BIX, RG and doxycycline i.c.v injections and VPA oral gavages for another 7 consecutive days and OBiBaRV; Oct4 expressing lentivirus i.c.v injection followed by BIX, Bay k, RG and doxycycline i.c.v injections and VPA oral gavages for another 6 consecutive days. Each group contains 5-7 animals. ***; P<0.001 compared to Vehicle group and ###; P<0.001 compared to OV group.