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. 2015 Jan 13;16(4):416–425. doi: 10.22074/cellj.2015.488

Fig 4.

Fig 4

Quantitative analysis of pluripotency and neural stem cell (NSC) markers following exogenous Oct4 expression along with or after VPA treatment. A. Endogenous Oct4 expression, B. Nanog expression, C. Klf4 expression, D. c-Myc expression, E. Sox2 expression, F. Pax6 expression and G. Sox1 expression in different groups. Results were normalized by GAPDH as a housekeeping gene. Vehicle; 7 days PBS i.c.v injection, V+Vehicle & Dox; VPA oral gavages for 7 days prior to PBS and doxycycline i.c.v injection for another 7 days, OV; Oct4 expressing lentivirusi.c.v injection followed by doxycycline i.c.v injections and VPA oral gavages for another 7 consecutive days and V+O; VPA oral gavages for 7 days prior to Oct4 expressing lentivirus i.c.v injection followed by doxycycline i.c.v injections for another 7 consecutive days. Each group contains 5-7 animals. **; P<0.01 and ***; P<0.001.