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. 2015 Jan 13;16(4):546–553. doi: 10.22074/cellj.2015.499

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Fig 6 — Photographs present in vitro follicle development. A. Preantral follicle at day 1 of culture: central germinal vesicle (GV)-stage oocyte with a thin zona, surrounded by granulosa cells. There are some theca cells and a basal membrane attached to the membrane.

B. At day 6 of culture, a very diffuse pattern of a growing follicle with a centrally located oocyte is spreading over the monolayer. An antrum-like cavity is formed in some follicles.

C. At day 7, a muciﬁed cumulus-oocyte complex ﬂoating free in the culture droplet is produced 16-18 hours post-human chorionic gonadotropin (hCG) stimulation.

D. The ovulated oocyte exhibits cumulus expansion and polar body extrusion.