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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Nature. 2014 Oct 26;517(7534):377–380. doi: 10.1038/nature13853

Extended Data Figure 1.

Extended Data Figure 1

Interaction between WUS/WOX and HAM family transcriptional regulators. (a) LacZ activity in yeast-two-hybrid assays testing interactions between WUS and HAM2, HAM3 or HAM4. Error bar = mean ± sem (n=3biological replicates). **, P<0.01; ***, P<0.001(two-tailed t-test, compared to DBD-WUS/AD). (b–o) Bimolecular fluorescence complementation (BiFC) analyses in tobacco transient assays with HAM and WOX family genes. Tobacco was co-transformed with GFPn-WUS and GFPc-HAM3 (b), or GFPn-WUS and GFPc-HAM4 (c), or GFPn-WUS and GFPc-FAMA (d), or GFPn-WUS and GFPc-BARD1 (e), or GFPn-WOX4 and GFPc-HAM1 (f), or GFPn-WOX4 and GFPc-FAMA (g), or GFPn-FAMA and GFPc-HAM1 (h), or GFPn-WOX5 and GFPc-HAM1 (i), or GFPn-WOX5 and GFPc-HAM2 (j), or GFPn-WOX5 and GFPc-HAM4 (k), or GFPn-WOX5 and GFPc-FAMA (l), or GFPn-WOX5 and GFPc-BARD1 (m), or GFPn-BARD1 and GFPc-HAM1 (n), or GFPn-BARD1 and GFPc-HAM2 (o), or GFPn-BARD1 and GFPc-HAM4 (p), or GFPn-FAMA and GFPc-HAM4 (q). BARD1 and FAMA proteins are both included as negative controls. Left panel: GFP channel; middle panel: propidium iodide (PI) staining channel; right panel: merged channels. Scale bar = 20 μm.