Table 3. Quantification of c-fos immunoreactivity in control (Ctrl) and conditional (Dlx-DOR) mice following novelty suppressed feeding.
Ctrl and Dlx-DOR mice were sacrificed 90 min after the NSF test. c-fos protein labeling is obtained by immunocytochemistry on brain sections. The quantification is performed on images acquired on the Hamamatsu scanner and expressed in number of c-fos positive cells per mm2. The level of c-fos positive cells in the Cg Cx, four subregions of the striatum, BMA and VTA is similar in the two groups. However, the quantification reveals a significant reduction of c-fos positive cells in the BLA, CeA and insular cortex on sections from Dlx-DOR in comparison with the Ctrl mice, whereas a significant increase is found in the NAc. n = 6-9 animals per genotype / 4-12 sections per regions / 2 counts per sections.
| Region | Bregma | c-fos positive cells / mm2 | Stats | ||
|---|---|---|---|---|---|
|
| |||||
| Ctrl | Dlx-DOR | ||||
| Nucleus accumbens | NAc | 1.18 | 391.0 ± 38.4 | 497.3 ± 43.2* | p < 0.05 |
| Cingulate cortex | Cg Cx | 1.10 | 986.9 ± 60.3 | 870.2 ± 85.4 | p > 0.05 |
| Insular cortex | Ins Cx | 1.10 | 237.1 ± 22.2 | 165.4 ± 11.9* | p < 0.05 |
| Striatum | 1.10 | ||||
| Dorsomedial | DM | 492.2 ± 95.1 | 448.9 ± 77.5 | p > 0.05 | |
| Dorsolateral | DL | 228.1 ± 50.2 | 253.1 ± 55.3 | p > 0.05 | |
| Ventromedial | VM | 168.0 ± 14.6 | 151.9 ± 9.9 | p > 0.05 | |
| Ventrolateral | VL | 74.6 ± 21.6 | 79.4 ± 19.3 | p > 0.05 | |
| Amygdala | -1.46 | ||||
| Basolateral nucleus | BLA | 228.3 ±26.4 | 138.7 ± 14.1** | p < 0.01 | |
| Basomedial nucleus | BMA | 323.0 ± 65.9 | 232.6 ± 21.4 | p > 0.05 | |
| Central nucleus | CeA | 298.6 ±33.6 | 153.0 ± 12.0*** | p < 0.001 | |
| Ventral Tegmental Area | VTA | -2.54 | 274.17 ± 28.32 | 352.38 ± 33.25 | p > 0.05 |
, P<0.05;
two stars: , P<0.01;
three stars: , P<0.001 (Student t-test).