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. Author manuscript; available in PMC: 2016 Jan 30.
Published in final edited form as: J Mol Biol. 2014 Nov 15;427(2):454–467. doi: 10.1016/j.jmb.2014.11.010

FIGURE 2. Changes in ensemble FRET between domains IV and II of EF-G induced by nucleotide and ribosome binding.

FIGURE 2

Increases or decreases in FRET efficiency relative to ribosome-free and nucleotide-free EF-G (ΔE=Ecomplex-Efree) are shown upon GTP binding (a); GDP binding (b), binding to the 70S ribosome containing deacylated tRNAMet in the P site and N-acetyl-Met-Phe-tRNAPhe in the A in the presence of GTP and fusidic acid (c); binding to the 70S ribosome containing deacylated tRNAMet in the P site and N-acetyl-Met-Phe-tRNAPhe in the A site, in the presence of GTP, fusidic acid and viomycin (d). Ensemble FRET measurements were taken for EF-G_301-603(Cy3/Cy5), EF-G_301-541(Cy3/Cy5), and EF-G_401-538(Cy3/Cy5). Error bars show standard deviations for changes in FRET efficiency were calculated from triplicate measurements.