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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell. 2014 Dec 24;160(0):74–87. doi: 10.1016/j.cell.2014.12.011

Figure 2. IL-33 stimulates proliferation and commitment of adipocyte precursors to the beige fat lineage.

Figure 2

(A, B) Quantification of ILC2 numbers (A) and activation status (B) in the scWAT of thermoneutral, heterozygous Red5 (Il5Red5/+) mice that were administered vehicle (Veh) or IL-33 for 8 days. Expression of IL-5 (td Tomato) from the Red5 allele was used as a marker of ILC2 activation (n=9–10 per treatment). (C) Quantification of eosinophils in the scWAT of thermoneutral heterozygous Red5 (Il5Red5/+) mice that were administered Veh or IL-33 for 8 days (n=8–10 per treatment). (D) Quantification of adipocyte precursor (AP) proliferation in the scWAT of thermoneutral Il5Red5/+ mice administered Veh or IL-33 for 8 days, as assessed by intracellular staining for Ki67 (D) and AP cell number per fat pad (n=8–10 per treatment). (F, G) Expression of beige adipocyte markers TMEM26 and CD137 on the scWAT APs of thermoneutral Il5Red5/+ mice administered Veh or IL-33 for 8 days (n=8–10 per treatment). Representative histograms for TMEM26 (F) and CD137 (G) are shown; clear histogram-Veh, shaded histogram-IL-33. (H–J) Expression of IL1RLI (H), IL-5Rα (I), and IL-4Rα (J) on scWAT APs of mice. For IL1RL1 (H), the clear histogram represents WT APs, while the shaded represents Il1rl1−/− APs. For IL-5Rα (I), the dashed line histogram represents isotype, the solid line represents APs stained for IL-5Rα, and the shaded histogram represents eosinophils stained for IL-5Rα. For IL-4Rα (J), the solid line histogram represents isotype and the shaded histogram represents APs stained for IL-4Rα. (K, L) Quantification of IL-33 induced AP proliferation in the scWAT of 114/13−/− (K) and Il4ra−/− (L) mice (n=4–8 per genotype and treatment). (M) Immunoblotting for UCP1 in the scWAT and BAT of thermoneutral WT and Il4ra−/− mice administered IL-33 for 8 days (n=2–3 per genotype and treatment). (N–P) Quantification of AP proliferation (N), TMEM26 (O) and CD137 (P) expression in Rag2−/− and Rag2−/− Il2rgc−/− mice treated with IL-33 (n=6–8 per genotype and treatment). Data are represented as mean ± SEM. See also Figure S1 and S2.