Skip to main content
. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2014 Dec 15;566:49–57. doi: 10.1016/j.abb.2014.12.007

Fig. 7. Effects of miR-424 on the expression of Smurf2.

Fig. 7

(A) 3′-UTR reporter assay. 3′-UTR reporter plasmids of Smad7, Smurf1 or Smurf2 were co-transfected with a miR-424 expression vector, or its control vector (Con), into HEK293 cells. The dual luciferase activities were measured and expressed a ratio of firefly to Renilla luciferase activity. The results were normalized to the empty vector control without 3′-UTR (EVC) from the two independent experiments, each with 3 replications. *P<0.005 v.s. control. (B-D) A549 cells were infected with a miR-424 lentivirus at a MOI of 50 for 48 h. Protein and mRNA levels of Smad7, Smurf1 and Smurf2 were determined using Western blotting and real-time PCR. (B) Western blot showed the protein expressions of Smurf1, Smurf2 and Smad7. GAPDH was used as a loading control. (C) The quantitation of protein levels from Western blotting using Image J. Data was normalized to GAPDH. (D) mRNA levels of Smurf1, Smurf2 and Smad7 were determined by real-time PCR and normalized to 18S rRNA. BC: Blank control, VC: Virus control. The results shown are means ± s.e. (n=3). *P<0.05 v.s. VC. Two-way ANOVA with bonferroni post-test.

HHS Vulnerability Disclosure