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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Clin Cancer Res. 2014 Nov 25;21(2):303–311. doi: 10.1158/1078-0432.CCR-14-1781

Figure 3. Sequential MMC + BCG polarizes TAMs towards an M1 phenotype.

Figure 3

Following treatment under different conditions, bladder cells were stained for flow and sorting. A. Expression analysis by qPCR of il10, arg1, and il6 mRNA and mean fluorescence intensity (MFI) of MHC was performed on CD45+CD11b+Gr-1- TAMs after gating on live singlets (mouse bladders pooled within each treatment group). B. Expression analysis of cxcl1 by qPCR on CD45+CD11b+Gr-1- TAMs.