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Following treatment under different conditions, bladder cells were stained for flow and sorting. A. Expression analysis by qPCR of il10, arg1, and il6 mRNA and mean fluorescence intensity (MFI) of MHC was performed on CD45+CD11b+Gr-1- TAMs after gating on live singlets (mouse bladders pooled within each treatment group). B. Expression analysis of cxcl1 by qPCR on CD45+CD11b+Gr-1- TAMs.
