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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Cytokine. 2014 Dec 5;71(2):289–295. doi: 10.1016/j.cyto.2014.11.006

Figure 1. tBHQ treatment induces expression of the Nrf2 target genes NQO1, GCLC, and HMOX as well as NRF2 expression in PBMCs.

Figure 1

PBMCs were either left untreated (Bkg), or pretreated with vehicle (Veh, 0.01% ethanol) or tBHQ (1–5µM) and then activated 30 min later with anti-CD3/anti-CD28. Total RNA was isolated after 6h and mRNA expressions of (A) NQO1, (B) GCLC, (C) HMOX, and (D) NRF2 were quantified by quantitative RT-PCR. * indicates p<0.05 compared to the Veh group.