Skip to main content
. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Cytokine. 2014 Dec 5;71(2):289–295. doi: 10.1016/j.cyto.2014.11.006

Figure 5. tBHQ treatment inhibits the expression of the early cell surface markers CD25 and CD69 in isolated CD4+ T cells.

Figure 5

CD4 T cells were either left untreated (Bkg), or pretreated with vehicle (Veh, 0.01% ethanol) or tBHQ (0.1–5µM) and then activated 30 min later with anti-CD3/anti-CD28. Cells were collected after 24h and labeled with fluorochrome-conjugated antibodies against CD25 and CD69. CD4+ T cells were gated prior to analysis of CD25 and CD69 expression. A) Representative dot plots and quantification of (B) CD25 and (C) CD69 protein expression. * indicates p<0.05 compared to the Veh group.