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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Cytokine. 2014 Dec 5;71(2):289–295. doi: 10.1016/j.cyto.2014.11.006

Figure 6. tBHQ treatment inhibits NFκB DNA binding in PBMCs.

Figure 6

PBMCs were left untreated (Bkg), or pretreated with vehicle (Veh, 0.01% ethanol) or tBHQ (1µM), then activated 30 min later with anti-CD3/anti-CD28. 3h after activation, cells were collected and nuclear protein was isolated. p65 NFκB DNA binding activity was quantified spectrophotometrically by measuring absorbance at 450 nm.