| Cycle | Temperature | Time |
|---|---|---|
| 1 | 95 °C | 3 min |
| 2 to 31 | 94 °C | 1 min |
| 57 °C | 1 min | |
| 72 °C | 45 s + 0.02 min cycle−1 | |
| 32 | 72 °C | 7 min |
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