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. Author manuscript; available in PMC: 2015 Jan 18.
Published in final edited form as: Microbiol Spectr. 2014 Sep 19;2(5):8. doi: 10.1128/microbiolspec.PLAS-0008-2013

FIG. 3.

FIG. 3

Genetic organization of the MOB cassette in pMV158. Plasmid-oriT and the mobM gene are flanked by two lagging-strand origins of replication (ssoA and ssoU). Bothssos have the possibility of generating long hairpin-loop structures exhibited as ‘ssDNA promoters’ (15, 18) where the RNAP-binding site (RSB) is recognized by the RNAP to synthesize a short RNA primer (pRNA). A consensus sequence (CS-6) located in the loop of the hairpin acts as the termination point for the pRNA synthesis. The pRNA is then used by DNA polymerase I for limited extension synthesis, followed by replication of the lagging strand by DNA Pol III.