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. Author manuscript; available in PMC: 2015 Jan 18.
Published in final edited form as: Microbiol Spectr. 2014 Sep 19;2(5):8. doi: 10.1128/microbiolspec.PLAS-0008-2013

FIG. 5.

FIG. 5

Enterococcus faecalis OG1RF expressing Green Fluorescent Protein (GFP) colonizes the intestine of C. elegans. L4 larvae were exposed for 3 hours to E. faecalis OG1RF carrying plasmid pMV158GFP. Subsequently, worms were washed with M9 medium and anesthetized with 0.25mM levamisol before imaging. DIC and fluorescence microscopy were used to visualize the worms. GFP expressing bacteria and auto-fluorescence generated by lipofuscin granules in the body of the worm were observed using FITC and DAPI filters respectively.